Figure 5. Long intergenic nonprotein-coding RNA 00514 (LINC00514) positively regulates URGCP in OS cells by sponging microRNA-708 (miR-708). (A, B) Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blotting were conducted to measure URGCP mRNA and protein levels, respectively, in LINC00514-depleted HOS and MG-63 cells. (C) URGCP mRNA expression in the 59 pairs of OS and adjacent normal tissue samples was determined by RT-qPCR. (D) Correlation between LINC00514 and URGCP mRNA expressions in OS tissues was analyzed by Spearman’s correlation analysis (r = 0.6105, P < 0.0001). (E) Efficiency of in-miR-708 expression transfection in HOS and MG-63 cells was evaluated by RT-qPCR. (F, G) HOS and MG-63 cells were transfected with si-LINC00514 together with in-miR-708 or in-miR-NC. After transfection, the mRNA and protein levels of URGCP were determined by RT-qPCR and western blotting, respectively. *P < 0.05 and **P < 0.01.