Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 8 pp 6793—6807
LINC00514 drives osteosarcoma progression through sponging microRNA-708 and consequently increases URGCP expression
Figure 7. Reintroduction of URGCP reverses the si-long intergenic nonprotein-coding RNA 00514 (LINC00514)-mediated inhibition of the malignant phenotype of OS cells. (A) URGCP protein expression in HOS and MG-63 cells after pc-URGCP or pcDNA3.1 transfection was detected by western blotting. (B–F) LINC00514-silenced HOS and MG-63 cells were further transfected with pc-URGCP or empty pcDNA3.1 plasmid. Cell proliferation, colony formation, and cell apoptosis and cell cycle status were assessed by Cell Counting Kit-8 assay, colony formation assay, and flow cytometry, respectively. (G, H) The migratory and invasive abilities of the aforementioned cells were assessed by transwell migration and invasion assays. *P < 0.05 and **P < 0.01.