Research Paper Volume 12, Issue 8 pp 6793—6807

LINC00514 drives osteosarcoma progression through sponging microRNA-708 and consequently increases URGCP expression

Figure 7. Reintroduction of URGCP reverses the si-long intergenic nonprotein-coding RNA 00514 (LINC00514)-mediated inhibition of the malignant phenotype of OS cells. (A) URGCP protein expression in HOS and MG-63 cells after pc-URGCP or pcDNA3.1 transfection was detected by western blotting. (BF) LINC00514-silenced HOS and MG-63 cells were further transfected with pc-URGCP or empty pcDNA3.1 plasmid. Cell proliferation, colony formation, and cell apoptosis and cell cycle status were assessed by Cell Counting Kit-8 assay, colony formation assay, and flow cytometry, respectively. (G, H) The migratory and invasive abilities of the aforementioned cells were assessed by transwell migration and invasion assays. *P < 0.05 and **P < 0.01.