Research Paper Volume 12, Issue 8 pp 6793—6807

LINC00514 drives osteosarcoma progression through sponging microRNA-708 and consequently increases URGCP expression

Figure 8. Decreased long intergenic nonprotein-coding RNA 00514 (LINC00514) expression attenuates tumor growth of OS cells in vivo. (A) Representative image of subcutaneous tumor xenografts collected from the sh-LINC00514 and sh-NC groups. (B) Tumor width and length was detected every 4 days for 1 month. Growth curve was plotted using the measurement. (C) One month after cell injection, all mice were sacrificed and subcutaneous tumor xenografts were weighed. (D, E) Quantitative reverse transcription polymerase chain reaction was performed to analyze LINC00514 and microRNA-708 expressions in the excised tumor xenografts. (F) URGCP protein level in the excised tumor xenografts was quantified by western blotting. **P < 0.01.