Figure 3. FAM84B regulates the proliferation, apoptosis, mitochondrial function and glycolysis of PDAC cells. (A) GSEA analysis revealed that FAM84B expression was negatively correlated with apoptosis, but positively correlated with glycolysis in TCGA PDAC dataset. NES: normalized enrichment score. (B) Western blotting analysis of FAM84B knockdown efficiency in AsPC-1 and CFPAC1 cell lines. (C) CCK-8 proliferation assay indicated that FAM84B knockdown decreased the growth of AsPC-1 and CFPAC1 cells. (D) Flow cytometry analysis indicated that FAM84B knockdown induced apoptosis of AsPC-1 and CFPAC1 cells. (E) Knockdown of FAM84B significant decreased extracellular acidification rates (ECAR). (F) Knockdown of FAM84B significantly decreased oxygen consumption (OCR) in AsPC-1 and CFPAC1 cells. (G) Knockdown of FAM84B significantly decreased 2-NBDG uptake. (H) Knockdown of FAM84B significantly decreased lactate production. NC: control siRNA; #1, #2, #3: FAM84B siRNA#1, #2, #3. *P<0.05, **P < 0.01 and ***P<0.001 vs. NC.