Research Paper Volume 12, Issue 8 pp 6808—6822

FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway

Figure 5. Effect of FAM84B knockdown on tumor progression in vivo. Nude mice were transplanted with AsPC-1 cells stably expressing control siRNA (NC) or FAM84B siRNA (siRNA#1). (A) Tumor volume were recorded for 33 days. (B) At 33 days post transplantation, xenografts were collected, photographed and weighed. Scale bar: 2 cm. (C) TUNEL assay was performed to assess apoptotic rate in the collected xenografts (magnification scale bar, 100 μm). (D) Nuclear β-catenin and the protein levels of c-Myc, LDHA and Survivin were detected. *P < 0.05 and ***P<0.001.