Research Paper Volume 12, Issue 8 pp 6808—6822

FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway

Figure 6. FAM84B expression affects PDAC cell sensitivity to gemcitabine. (A, B) AsPC-1 and PANC-1 cells were exposed to 20 μM gemcitabine or Vehicle (DMSO) for 12, 24 and 48 h (A). AsPC-1 and PANC-1 cells were exposed to 5, 20 and 50 μM gemcitabine or Vehicle (DMSO) for 48 h (B). CCK-8 assay was performed to assess the inhibition rate of cell proliferation. (CE) Nude mice bearing xenografts formed from AsPC-1 and PANC-1 cells were treated with gemcitabine (GEM) or Vehicle (DMSO) (n=20 each group). Inhibition of tumor growth (C) were shown. At 21 days after treatment with GEM or Vehicle, 5 mice of each group were sacrificed, and the xenografts were collected, photographed and weighed (D). Scale bar: 2 cm. Survival analysis was performed on the remaining mice for 90 days (n=15 each group). *P<0.05, **P<0.01, and ***P<0.001.