Figure 1. High expression of hsa_circ_0072995 (circRGNEF) predicted an unfavorable prognosis of bladder cancer (BC). (A) RT-qPCR assay of circRGNEF in 90 paired BC tumor and adjacent non-tumor tissues. Data are means ± standard deviation (SD). ***P < 0.001 vs. normal controls. (B) Fluorescent in situ hybridization (FISH) indicates the subcellular localization of circRGNEF. (C) The prognostic significance of circRGNEF expression for BC patients was performed with FISH values using the median value as the cut-off. The observation time was 60 months. (D) Genomic loci of RGNEF and circRGNEF. The red signal indicates back splicing.