Research Paper Volume 12, Issue 8 pp 6865—6879

Circular RNA circRGNEF promotes bladder cancer progression via miR-548/KIF2C axis regulation

Figure 6. Downregulation of miR-548 or overexpression of KIF2C restored proliferation, migration, and invasion after circRGNEF silencing. (AF) RT-qPCR shows the expression of circRGNEF (A, B), miR-548 (C, D), and KIF2C (E, F) in T24 and UM-UC-3 cells following transfection or treatment with NC, si-circRGNEF, miR-548 inhibitor, KIF2C overexpression vector (KIF2C) single or combined. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. si-circRGNEF. (GI) Cell proliferation was analyzed by EdU assays. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. si-circRGNEF. (JN) Cell migration and invasion were assessed in T24 and UM-UC-3 cells using Transwell assays. Data are presented as the mean ± SD. ***P < 0.001 vs. NC. ###P < 0.001 vs. si-circRGNEF.