Research Paper Volume 12, Issue 8 pp 6880—6890

PP2A subunit PPP2R2C is downregulated in the brains of Alzheimer’s transgenic mice

Figure 1. PPP2R2C regulates PP2A phosphatase activity. (A) Phosphatase activity combined with immunoprecipitation of PP2A was measured in SHSY5Y human neuroblastoma cell lines. Cells were either knocked down PPP2R2C expression by shRNA or overexpressed wildtype PPP2R2C by pWPIR-GFP lentivirus vector. Data are shown in mean+/-SEM, n=3, *p<0.05, ***p<0.001. (B) Representative image of immunoblots of indicated antibodies are shown in SHSY5Y cell lines after knockdown and overexpression PPP2R2C. (C) Quantification of the intensities of the protein bands from three independent immunoblots of (B). Data are shown in mean+/-SEM, n=3, *p<0.05.