Research Paper Volume 12, Issue 8 pp 6891—6903

Hypoxia-induced let-7f-5p/TARBP2 feedback loop regulates osteosarcoma cell proliferation and invasion by inhibiting the Wnt signaling pathway

Figure 2. let-7f-5p suppressed proliferation and invasion of Saos cells in vitro and in vivo. (A) The CCK8 assay results showed that let-7f-5p suppressed the proliferation of Saos cells in vitro. (B) The colony formation assay results showed that let-7f-5p suppressed the clonality of Saos cells in vitro. (C) The Transwell assay results showed that let-7f-5p suppressed the invasion of Saos cells in vitro. (D) Representative graphs and statistical results of tumor masses harvested 4 weeks after injecting Saos cells subcutaneously into nude mice. (E) Representative photographs of lung metastases and statistical results of lung metastatic nodule numbers between the Saos-let-7f-5p-o and Saos-control groups 6 weeks after injecting Saos cells into the lateral tail veins of nude mice (magnification: 100× and 200×).