Research Paper Volume 12, Issue 8 pp 6891—6903

Hypoxia-induced let-7f-5p/TARBP2 feedback loop regulates osteosarcoma cell proliferation and invasion by inhibiting the Wnt signaling pathway

Figure 6. The hypoxia-induced let-7f-5p/TARBP2 feedback loop inhibited the Wnt signaling pathway in OS cells. (A, E) Western blot analyses of the protein expression levels of β-catenin, Axin2, p-GSK3β, and GSK3β in Saos cells under normoxia or hypoxia conditions. (B, F) Western blot analyses of the protein expression levels of β-catenin, Axin2, p-GSK3β, and GSK3β in Saos cells following transfection with TARBP2 siRNA or control oligonucleotide. (C, G) Western blot analyses of the protein expression levels of β-catenin, Axin2, p-GSK3β, and GSK3β in Saos cells following transfection with the let-7f-5p inhibitor or control oligonucleotide. (D, H) Wnt inhibitor could abolish TARBP2 siRNA and let-7f-5p inhibitor-induced Wnt signal pathway activation. (I) β-catenin is mainly located in the cytoplasm, and hypoxia promoted the transfer of β-catenin to the nucleus.