Research Paper Volume 12, Issue 8 pp 6904—6927

Identification of UAP1L1 as tumor promotor in gastric cancer through regulation of CDK6

Figure 6. UAP1L1 knockdown inhibited gastric cancer development in vivo. (A) 7 days post injection of SGC-7901 cells with or without UAP1L1 knockdown, the volume of tumors formed in mice was measured and calculated at indicated time intervals. (B) In vivo imaging was performed to evaluate the tumor burden in mice of shUAP1L1 and shCtrl groups at day 22 post tumor-inoculation. (C) The bioluminescence intensity was scanned and used as a representation of tumor burden in mice of shUAP1L1 and shCtrl groups. (D, E) Mice were sacrificed at day 22 post injection, and the tumors were removed for collecting photos (D) and weighing (E). (F, G) The expression of UAP1L1, CDK6, E-cadherin and ZO-1 in xenografts was detected by western blotting. Data was shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.