Research Paper Volume 12, Issue 8 pp 6947—6965

Diabetes-induced glucolipotoxicity impairs wound healing ability of adipose-derived stem cells-through the miR-1248/CITED2/HIF-1α pathway

Figure 6. MiR-1248 regulated HIF-1α via targeting CITED2 under hypoxia. (A) Schematic diagram of the CITED2 putative binding sites in miR-1248; (B) The mRNA expression of CITED2 while miR-1248 mimics or inhibition in the different hADSCs was determined by RT-qPCR; (C) The protein expression of CITED2 while miR-1248 mimics or inhibition in the different hADSCs was determined by western blot; (D) The luciferase reporter assay to verify the influence of miR-1248 on CITED2 expression; (E) The expression of the CITED2 was detected in the three hADSCs by western blot; (F) The protein expression of CITED2 was estimated in the different hADSCs by immunofluorescence; (G) The protein expression of the HIF-1α was detected in the different hADSCs under anoxia or normoxia by western blot; (H) The expression of the HIF-1α was detected in the different hADSCs under anoxia by immunofluorescence; (I) The expression of angiogenesis related genes (including HIF-1α, VEGFα, CXCR4, MMP2, FGF2 and TGF-β) under the anoxia or normoxia were examined by RT-qPCR (* P<0.05).