Research Paper Volume 12, Issue 8 pp 7042—7055

SIRT1 regulates O-GlcNAcylation of tau through OGT

Figure 3. SIRT1 inhibits OGT expression. HEK-293A cells were transfected with pcDNA3.1, pcDNA3.1/Myc-SIRT1 or pcDNA3.1/Myc-H363Y. (A) mRNA levels of OGT and GAPDH were measured by RT-PCR. (B) The quantification of relative mRNA level of OGT after normalization with the mRNA level of GAPDH was represented as mean ± S.D. (n = 3); ***, p < 0.001. (C) Protein levels of Myc-SIRT1 or Myc-H363Y were analyzed by western blot developed with anti-Myc antibody. GAPDH was used as the loading control. (D) Blot shown in panel C was quantified for protein expression levels of OGT after being normalized with GAPDH level. Data are presented as mean ± S.D. (n=3), **, p < 0.01.