Figure 7. SIRT1 deacetylates CREB and inhibits phosphorylation of CREB at Ser133. (A) pCI/HA-CREB was co-transfected with pcDNA3.1/SIRT1 or pcDNA3.1/H363Y into HEK-293A cells. CREB was immunoprecipitated with anti-HA and analyzed by western blots developed with anti-CREB or anti-acetylated-lysine or anti-pS133-CREB antibody. The relative levels of CREB acetylation (B) or phosphorylation at Ser133 (C) are normalized with CREB levels and are represented as mean ± S.D. (n = 3); *, p < 0.05, **, p < 0.01, ***, p < 0.001.