Research Paper Volume 12, Issue 8 pp 7183—7206

Integrated data analysis reveals significant associations of KEAP1 mutations with DNA methylation alterations in lung adenocarcinomas

Figure 3. Differential methylation analysis of samples from KEAP1-mutated LUAD patients vs normal samples. (A) Graphic showing 450k DNA methylation analysis. (a) Graphic showing percentage of differentially-methylated CpG sites in KEAP1-mutated LUAD patients compared to normal samples. (b) Percentages of hypomethylation and hypermethylation. (c) Distribution of hypermethylated CpG sites in KEAP1-mutated patient samples according to functional genomic distribution, neighborhood context, associated RNA transcripts, and chromosomal location. (d) Distribution of hypomethylated CpG sites in KEAP1-mutated patient samples according to functional genomic distribution, neighborhood context, associated RNA transcripts, and chromosomal location. (B) Heatmap showing the differentially-methylated CpG sites in KEAP1-mutated LUAD patients compared to normal individuals (delta β >|0.4|, p <0.05 with BH-FDR adjustment). Methylation Beta-values are represented as row Z-score. The blue color indicates decreased CpG methylation while the pink color indicates increased CpG methylation. The heatmap was generated using the ClustVis webtool.