Figure 5. In silico analysis of NRF2 binding sites. (A) Bar graph showing the top 10 enriched transcription factor perturbation followed by expression datasets (highest p value) after the input of the 80 differentially methylated genes using enrichr database. 5 data sets where NRF2 was Perturbed among the top 10 enriched terms (highest p value). (B) Heatmap showing the differentially-methylated genes (20 genes) that were enriched in any of the top 10 enriched terms. The heatmap shows 15 out of these 20 genes enriched in 5 datasets where NRF2 was Perturbed. (C) The NRF2 binding motif as provided by JASPER. (D) Schematic representation of the locations of in silico-predicted NRF2 binding sites (AREs) in the promoter regions of the human ACOT7, LNOP2, SCNN1A, and BRF2 genes.