Research Paper Volume 12, Issue 8 pp 7248—7261

MicroRNA-375 exacerbates knee osteoarthritis through repressing chondrocyte autophagy by targeting ATG2B

Figure 3. The effects of miR-375 and ATG2B on ERs. (A) Western blot of p-eIF2a and CHOP after infected with miR-375 mimics, miR-375 inhibitor, pcDNA3.1-ATG2B and pcDNA3.1-ATG2B plus miR-375 mimics in OA chondrocytes; (B) Qualitative analysis of p-eIF2a and CHOP, and the values were normalized to β-actin; (C, D) OA chondrocytes were double stained with CHOP (red) and DAPI (blue) and visualized by confocal microscopy after different treatment. ** P<0.01, compared with NC group; #, P<0.05, compared with pcDNA3.1-ATG2B group.