Figure 2. HMGN5 interacts with Hsp27 in vivo and in vitro. (A) The protein levels of Hsp27 and HMGN5 in bladder cancer cell lines examined by immunoblotting. (B) The protein levels and localization of Hsp27 and HMGN5 in T24 and J82 cells examined by IF staining. (C) In vitro GST pull-down assays were used to examine the interaction between HMGN5 and Hsp27.