Research Paper Volume 12, Issue 8 pp 7282—7298

HMGN5 promotes IL-6-induced epithelial-mesenchymal transition of bladder cancer by interacting with Hsp27

Figure 4. Silencing of HMGN5 and Hsp27 inhibits bladder cancer cell invasion and EMT. (A) T24 cells were transduced with Lv-sh-HMGN5 or Lv-sh-Hsp27, separately, or cotransduced with Lv-sh-HMGN5 and Lv-sh-Hsp27, and examined for cell invasion by Transwell assays. (B) The protein levels of E-cadherin and Vimentin by immunoblotting. (C) The protein levels of E-cadherin and Vimentin by IF staining. The data are presented as the mean ± SD of three independent experiments. **P<0.01.