Research Paper Volume 12, Issue 8 pp 7313—7333

Estrogen regulation of germline stem cell differentiation as a mechanism contributing to female reproductive aging

Figure 7. Lack of effect of Esr1 gene disruption in testes of adult male mice. (A) Quantitative analysis of Stra8 mRNA levels in testes of wild type (WT) and Esr1–/–mice at 3 months of age (mean ± SEM; n = 3 mice per group). (B, C) Histological appearance of the testes of WT (B) and Esr1–/– (C) mice at 3 months of age, after fixation and analysis by immunohistochemistry for Stra8 expression (brown immunoreaction product against a blue hematoxylin counterstain).