Research Paper Volume 12, Issue 8 pp 7350—7362

Inhibition of stearoyl-coenzyme A desaturase 1 ameliorates hepatic steatosis by inducing AMPK-mediated lipophagy

Figure 2. Effects of inhibited SCD1 expression on lipid deposition and activation of AMPK and lipophagy in primary hepatocytes. (A, B) Screening for the appropriate siRNA-SCD1 by Western blotting. (C) TG levels were measured after transfection with siRNA-SCD1. (D) Primary hepatocytes were stained with Oil Red O. a, control group; b, siRNA-SCD1 group; c, PA group; d, PA+siRNA-SCD1 group. (E) The intracellular lipid content in each group was quantified. (F, G) Protein levels were determined by Western blotting. The data are presented as the means±SDs. *p < 0.05 versus control, #p < 0.05 versus the PA group.