Figure 3. Effects of SCD1 over-expression on lipid deposition and activation of AMPK and lipophagy in primary hepatocytes. (A, B) The effect of SCD1-OE infection was verified by Western blotting. (C) TG levels were measured after infection with SCD1-OE. (D) Primary hepatocytes were stained with Oil Red O. a, control group; b, SCD1-OE group; c, PA group; d, PA+SCD1-OE group. (E) The intracellular lipid content in each group was quantified. (F, G) Protein levels were determined by Western blotting. The data are presented as the means±SDs. *p < 0.05 versus control, #p < 0.05 versus the PA group.