Figure 4. Effects of cotreatment with siRNA-SCD1 and the AMPK inhibitor on lipid deposition and lipophagy in primary hepatocytes. (A) TG levels were measured. (B) Primary hepatocytes were stained with Oil Red O. a, control group; b, siRNA-SCD1 group; c, Dorsomophin group; d, siRNA-SCD1+Dorsomophin group; e, PA group; f, PA+siRNA-SCD1 group; g, PA+Dorsomophin group; h, PA+siRNA-SCD1+Dorsomophin group. (C) The intracellular lipid content in each group was quantified. (D, E) Protein levels were determined by Western blotting. (F) Autophagosomes and autolysosomes in hepatocytes were observed by TEM. The red arrow indicates autophagosomes and autolysosomes. The data are presented as the means±SDs. *p < 0.05 versus control, #p < 0.05 versus the siRNA-SCD1 group, **p < 0.05 versus the PA group, ##p < 0.05 versus the PA+siRNA-SCD1 group.