Figure 5. Effects of cotreatment with SCD1-OE and the AMPK activator on lipid deposition and lipophagy in primary hepatocytes. (A) TG levels were measured. (B) Primary hepatocytes were stained with Oil Red O. a, control group; b, SCD1-OE group; c, A-769662 group; d, SCD1-OE+A-769662 group; e, PA group; f, PA+SCD1-OE group; g, PA+A-769662 group; h, PA+SCD1-OE+A-769662 group. (C) The intracellular lipid content in each group was quantified. (D, E) Protein levels were determined by Western blotting. (F) Autophagosomes and autolysosomes in hepatocytes were observed by TEM. The red arrow indicates autophagosomes and autolysosomes. The data are presented as the means±SDs. *p < 0.05 versus control, #p < 0.05 versus the SCD1-OE group, **p < 0.05 versus the PA group, ##p < 0.05 versus the PA+SCD1-OE group.