Research Paper Volume 12, Issue 8 pp 7350—7362

Inhibition of stearoyl-coenzyme A desaturase 1 ameliorates hepatic steatosis by inducing AMPK-mediated lipophagy

Figure 6. Effects of in vivo inhibition of SCD1 expression on hepatic steatosis and activation of AMPK and lipophagy in mice. (A) The body weights of mice in each group. (B) Levels of serum liver enzymes in mice after 14 weeks (n=6). (C) Levels of serum lipids in mice after 14 weeks (n=6). (D) H&E staining of mouse hepatic tissue (original magnification: 100X and 400X). (E) Oil Red O staining of mouse hepatic tissue (original magnification: 100X and 400X). (F, G) Protein levels were determined by Western blotting. (H) Autophagosomes and autolysosomes in hepatocytes in the liver tissues of mice were observed by TEM. The red arrows indicate hepatocytes, the yellow arrows indicate lipid droplets, and the green arrows indicate autophagosomes and autolysosomes. The data are presented as the means±SDs. *p < 0.05 versus the NCD group, #p < 0.05 versus the HFD group.