Research Paper Volume 12, Issue 8 pp 7431—7447

Sirt1 is regulated by miR-135a and involved in DNA damage repair during mouse cellular reprogramming

Figure 2. Identification of the SIRT1 interacting proteins during initiation phase of reprogramming. (A) 2° MEFs containing DOX inducible MKOS was sorted out by FACS according to the FITC gating indicated by circle dash line. (B) co-immunoprecipitation assay using SIRT1 antibody or rabbit IgG followed by Western blotting analysis (IB) using antibodies against SIRT1, KU70 and WRN.