Figure 3. The effects of RSV on DNA damage markers during reprogramming. Immunocytochemistry analysis on expression of (A) γH2AX and (B) 53BP1 in 2° MEFs before (-DOX) and after (+DOX) treatment. (C) FACS analysis showing the effect of RSV on γH2AX expression. (D) The numbers of cells with more than five (>5 foci, white), three to five (3-5 foci, black), one to two (1-2 foci, grey) and no (no foci, stripped) 53bp1 foci without (+DOX) or with (+RSV) RSV treatment. (n=3; *: p<0.05 when compared to the same category; chi square test).