Figure 1. Expression of Nrk in VSMCs. (A) Expression of Nrk protein was determined by western blotting analysis in mVSMCs, rVSMCs (A10), hVSMCs, HUVECs, HCAECs, HPAECs, C2C12 and A549 cells. Primary antibodies against mNrk (upper panel) and hNrk (middle panel) were employed for the detection of Nrk. Actin was used as a loading control (lower panel). (B) Expression of mNrk in normal carotid artery of wild-type C57BL/6 mice was examined by immunohistochemical staining with primary antibodies against mNrk, CD31, αSMA, and elastic stain. Bar= 50 μM. (C) Expression and localization of αSMA (green) and mNrk (red) on mouse carotid artery was examined by double staining of immunofluorescence confocal microscopy.