Figure 2. Expression of Nrk was suppressed by PDGF and LPS in mVSMCs. mVSMCs were serum starved (0.5% FBS in DMEM) for 24 h, followed by stimulation with PDGF (10 ng/ml) or LPS (100 ng/ml) for an additional 24 h. Expression of mNrk was determined by (A) western blotting (n=6) and (B) qPCR analysis (n=4). Gene expression of qPCR analysis results were normalized to both control cells as well as to GAPDH. Tubulin was used as a loading control for western blotting analysis. Scale bars: means ± SD. *, p <0.05.