Figure 3. Expression of mNrk, αSMA, and elastic staining in carotid artery of wild-type C57BL/6 mice subjected to guide wire injury for 4 weeks. The expression of mNrk, αSMA, and elastic staining in three set of sections of (A) non-injured and (B) injured carotid arteries at 150-μm intervals was examined by immunohistochemical staining. Bar = 50 μM. (C) Left panel: Quantitation of intima/media (I/M) ratio (left panel, p = 0.00056) and Nrk expression (right panel, p = 1.184 × 10-5) in normal and injured carotid arteries. n = 9 for each group.