Research Paper Volume 12, Issue 8 pp 7511—7533

Expression of Nik-related kinase in smooth muscle cells attenuates vascular inflammation and intimal hyperplasia

Figure 6. Effect of resveratrol on LPS- and Nrk siRNA-stimulated MMPs and chemokines. (A) mVSMCs were serum starved (0.5% FBS in DMEM) for 24 h and then treated with LPS (100 ng/mL) and/or resveratrol (50 μM) for 24 h. Cells were further transfected with 20 nM of negative control or mNrk siRNA for an additional 48 h. Expression of (A) MMP3 (n=8), CCL8 (n=8), CCL11 (n=8), CXCL3 (n=6) and CXCL5 (n=6) was determined by qPCR. Gene expression results of qPCR analysis were normalized to both control cells as well as GAPDH. (B) Protein levels of MMP3 (n=7), CCL8 (n=6) and CCL11 (n=7) in cultured conditioned media were determined by ELISA (normalized to total protein concentration). Scale bars: means ± SD. *, p < 0.05, **, p < 0.01, ***, p<0.001.