Research Paper Volume 12, Issue 8 pp 7534—7548

Protective effects of autophagy and NFE2L2 on reactive oxygen species-induced pyroptosis of human nucleus pulposus cells

Figure 5. ROS-induced pyroptosis was NLRP3 and PYCARD dependent. (A) The western blot detecting the expression of cleaved CASP1 and PYCARD in the nucleus pulposus cells transfected with PYCARD-shRNA and non-targeting shRNA (NVC) before treatment with hydrogen peroxide. (B) The panel compared the data measured in Figure A. (C) The western blot detecting the expression of cleaved CASP1 and NLRP3 in the nucleus pulposus cells after transfection with NLRP3-shRNA and non-targeting shRNA (NVC) before treatment with hydrogen peroxide. (D) The panel compared the data measured in Figure C. (E) The hochest33342/PI double staining showed that the PI positive cells were decreased when NLRP3 or PYCARD was silenced before treatment with hydrogen peroxide. (magnification: ×10, scale bar = 200μm) The data were represented as mean ± SEM. *P < 0.05.