Research Paper Volume 12, Issue 8 pp 7534—7548

Protective effects of autophagy and NFE2L2 on reactive oxygen species-induced pyroptosis of human nucleus pulposus cells

Figure 7. Proposed model of the ROS-induced pyroptosis and the negative regulation in NPCs. By increasing the reactive oxygen species level of nucleus pulposus cells, hydrogen peroxide upregulated the expression of NLRP3 and PYCARD to promote the expression of cleaved CASP1, IL-1β and IL-18 and the pore-forming in the membrane. The increased reactive oxygen species also increased the autophagy and NFE2L2 which both attenuated reactive oxygen species induced pyroptosis of nucleus pulposus cells.