Research Paper Volume 12, Issue 11 pp 10235—10245

miR-195-5p alleviates acute kidney injury through repression of inflammation and oxidative stress by targeting vascular endothelial growth factor A

Figure 2. Overexpression of miR-195-5p reduced hypoxia-induced renal injury in NRK-52E cells. (A) miR-195-5p expression in NRK-52E cells transfected with miR-195-5p mimics, inhibitors, or the corresponding negative controls for 48 h. (B) The CCK-8 assay was carried out to test cell viability. (C and D) EdU assays were performed to detect cell proliferation. (E) Flow cytometry analysis was employed to test cell apoptosis. NRK-52E cells were transfected with miR-195-5p mimics or corresponding negative controls for 48 h. Three independent experiments were performed. Error bars represent the mean ± SD of at least three independent experiments. *P < 0.05.