Figure 5. VEGFA directly targeted miR-195-5p. (A) The binding correlation between miR-195-5p and VEGFA. (B) Luciferase reporter constructs containing the WT-VEGFA or MUT-VEGFA sequence. (C) WT-VEGFA or MUT-VEGFA was co-transfected into NRK-52E cells with miR-195-5p mimics or their corresponding negative controls. (D) VEGFA mRNA expression in NRK-52E cells transfected with miR-195-5p mimics. (E and F) VEGFA protein expression in NRK-52E cells. Three independent experiments were performed. Error bars represent the mean ± SD of at least three independent experiments. *P < 0.05.