Figure 6. VEGFA expression was downregulated in AKI. (A) Analysis of VEGFA mRNA expression in serum from healthy controls and patients with AKI. GAPDH served as a reference control. (B) Immunohistochemical staining of VEGFA in kidney tissues from I/R rats. (C–E) VEGFA expression in I/R rat models. (F) VEGFA mRNA expression in NRK-52E cells exposed to hypoxia for 6 h. Eight rats were used in each group. Three independent experiments were performed. Error bars represent the mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01.