Figure 7. Loss of VEGFA repressed renal injury in vitro and in vivo. (A) VEGFA mRNA expression. NRK-52E cells were transfected with miR-195-5p inhibitors and infected with LV-shVEGFA under hypoxic conditions. (B) The CCK-8 assay was used to evaluate cell survival. (C and D) EdU assay was performed to detect cell proliferation. (E) Flow cytometry was used to detect cell apoptosis. miR-195-5p inhibitors and LV-shVEGFA were injected into the rat via the tail vein prior to I/R surgery. SOD (F) and MDA (G) were evaluated in kidney tissues. IL-6 (H) and TNF-α (I) protein levels in kidney tissues from rats. Eight rats were used in each group. Three independent experiments were performed. Error bars represent the mean ± SD of at least three independent experiments. *P < 0.05.