Research Paper Volume 12, Issue 12 pp 11364—11385

Sirt1 gene confers Adriamycin resistance in DLBCL via activating the PCG-1α mitochondrial metabolic pathway

Figure 2. Upregulation of Sirt1 expression confers resistance to Adriamycin-induced apoptosis of Non-GCB DLBCL cells. (A) The corresponding lentivirus was used to treat each group of LY-10 cells. Positive lentivirus mediated Sirt1 transduction (>95%) was observed under fluorescence microscopy (Scale bars: 100μm). (B, C) CCK-8 assay was used to detect cell viability. (D) The corresponding lentivirus was used to treat each group of LY-3 cells. Positive lentivirus mediated Sirt1 transduction (>95%) was observed under fluorescence microscopy (Scale bars: 100μm). (E, F) CCK-8 assay was used to detect cell viability. All experiments were performed in triplicate. * indicates p<0.05 against control group.