Research Paper Volume 12, Issue 12 pp 11364—11385

Sirt1 gene confers Adriamycin resistance in DLBCL via activating the PCG-1α mitochondrial metabolic pathway

Figure 4. Upregulation of Sirt1 conferres Adriamycin resistance of DLBCL cells in vivo. (A) LY-10 cells (1×107 cells) were subcutaneously inoculated into the flanks of nude mice to establish a xenograft mouse model of DLBCL. The mice were treated twice a week with 100 mg/kg Adriamycin when the tumors were palpable (day 12). (B) After 4 weeks of treatment with Adriamycin, tumor growth was observed through live imaging of each group of mice. Representative images of tumor-bearing mouse cells treated with Adriamycin (100 mg/kg). (C) Tumors from all mice in the indicate cell together with the mean tumor weights. Hematoxylin-eosin staining method was used to observe microscopic images of tumor cells. A representative sample (Vector1: 4; Sirt1: 4; Vector2: 4; Si-sirt1: 4) is shown (200 ×). (D) Tumor volumes were measured on the days indicated. Data were analyzed using Prism v5.0 (GraphPad Software, San Diego, CA, USA). Each bar represents the mean ± SD of three independent experiments. * p<0.05.