Figure 7. Potential interaction mechanism of Sirt1 with PGC1-α in vitro. (A) IHC staining demonstrating the expression of TUNEL-positive cells in the indicated tissues is shown (200×). Each bar represents the mean ± SD of three independent experiments. * p<0.05. (B) LY-10 cells were treated with Adriamycin (0.5 μM) for 24 hours. The protein expression of Sirt1, PGC1-α and Ace-PGC1-α were detected using western blotting. Western blotting bands were quantified using Quantity One software. Each sample was normalized to the expression of β-actin. All experiments were performed in triplicate. * p<0.05, ** p<0.01. (C) Immunoprecipitation assay indicating that Sirt1 interacts with PGC1-α in LY-10 cells.