Figure 4. Effects of miR-623 on cell apoptosis. (A–C) The apoptosis of MDA-MB-453 and MCF7 cells was determined using double staining with annexin V/propidium iodide (PI) by flow cytometry. (D–G)The protein levels of apoptosis-related genes in MDA-MB-453 cells and MCF7 cells were detected by western blot assay. GAPDH was the internal control. Relative amounts of proteins normalized to GAPDH were shown. Experiments showing identical results were performed three times. *P<0.05. P values were determined using Student’s t-tests. *P<0.05 compared with O-NC. #P<0.05 compared with siNC.