Research Paper Volume 12, Issue 12 pp 11386—11397

Bone marrow mesenchymal stem cells improve thymus and spleen function of aging rats through affecting P21/PCNA and suppressing oxidative stress

Figure 1. The isolation and identification of BMSCs and directional differentiation of BMSCs to steatoblast and osteoblasts. (A) The isolation and culture of BMSCs; (B) Clone formation ability of BMSCs stained by crystal violet staining; (C) Induction of steatoblast stained by Oil Red O staining; (D) Induction of osteoblasts stained by alizarin red staining; (E) Identification of surface markers of BMSCs by flow cytometry. White arrows indicate clone formation; Black arrows indicate lipid vesicles; Green arrows indicate calcified nodules.