Research Paper Volume 12, Issue 12 pp 11431—11445

Chestnut polysaccharides benefit spermatogenesis through improvement in the expression of important genes

Figure 5. The expression of important proteins in spermatogenesis. (A) The network of important genes/proteins in spermatogenesis. (B) The protein levels of VASA, DAZL, PGK2, and CREM using Western blot analysis. (C) The relative abundance of VASA, DAZL, PGK2, and CREM in different groups, ns is stand for no difference, *P<0.05, **P < 0.01. (D) The immunofluorecence staining images of VASA and DAZL. (E) The quantitative data for VASA in immunofluorecence staining. The results are presented as mean ± SEM, **P < 0.01. (F) The quantitative data for DAZL in immunofluorecence staining. The results are presented as mean ± SEM, **P < 0.01.