Research Paper Volume 12, Issue 14 pp 14037—14049

Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma

Figure 4. CNN3 silencing induces apoptosis and G1-stage cell cycle arrest in vitro. (A) The effect of CNN3 silencing on apoptosis. Representative graphs of flow cytometric analysis for apoptosis are shown on top. In all four plots, viable cells are seen in the left lower quadrant (FITC-/PI-); early apoptotic cells in the right lower quadrant (FITC+/PI-); late apoptotic cells in the right upper quadrant (FITC+/PI+); and necrotic cells in the left upper quadrant (FITC-/PI+). Statistical results of the percentage of total apoptotic cells, which are the sum of early and late apoptotic cells are shown below. (B) The effect of CNN3 silencing on cell cycle. Graphs showing the flow cytometric analysis for cell cycle stage are on top. The statistical result of cells in G1, G2, and S stages is shown below. *p<0.05, when LV-shCNN3 vs LV-NC.