Research Paper Volume 12, Issue 12 pp 11446—11465

microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway

Figure 1. miR-9 and miR-29a are identified with high expression in DM, and both can bind to 3’UTR of ISL1. (A) The fold-change values of miRs calculated according to 16 included studies. Log2 Fold change of miR = Log2 (miR expression in DM) – Log2 (miR expression in NC). Fold change > 2 is indicative of upregulated miR in DM. Fold change < 0 is indicative of downregulated miR in DM. (B) The expression of ISL1 in microarray datasets GSE27382 (mouse sciatic nerves) and GSE95849 (human peripheral blood). (C) ISL1 was a putative target gene of both miR-9 and miR-29a in humans according to the target prediction program available on miRanda. (D) ISL1 was a putative target gene of both miR-9 and miR-29a in mice according to target prediction program available on miRanda. (E) ISL1 was a putative target gene of both miR-9 and miR-29a in rats according to target prediction program available on TargetScan. (F, G) The luciferase activity determination in HEK293T cells indicated that ISL1 was indeed a target gene of both miR-9 and miR-29a. (H) The expression of ISL1, miR-9 and miR-29a in sciatic nerves after streptozotocin induction on the 0 day determined by RT-qPCR. (I) The expression of ISL1, miR-9 and miR-29a in sciatic nerves after streptozotocin induction on the 3rd day determined by RT-qPCR (n = 8). Luciferase activity and gene expression were measurement data and expressed as mean ± standard deviation. Luciferase activity was analyzed by two-way analysis of variance; gene expression was analyzed by t test; the experiment was repeated three times independently; * p < 0.05 vs. the normal or NC group.