Figure 1. Modulation of AR influence the expression of PD-L1 in HCC cells. (A–C) RT-qPCR analysis of three checkpoints in over-expression AR and down-expression AR HCC cells. (D) Western Blot of AR and PD-L1 in three HCC cell lines. (C) Flow cytometry of the membrane PD-L1. (E, F) Mean fluorescence intensity of membrane PD-L1. *P<0.05, ** P<0.01 and *** P<0.001.