Research Paper Volume 12, Issue 12 pp 11466—11484

Androgen receptor affects the response to immune checkpoint therapy by suppressing PD-L1 in hepatocellular carcinoma

Figure 5. AR overexpression attenuated the effects of the PD-L1 inhibitor in vivo. (A) Establishment of overexpressed AR Hep1-6 and tested by western blot and flow cytometry. (B) Flow diagram of animal experiment. (C) The luminescence of tumor detected by IVIS system. (D) The growth curve of mice liver tumor represented by photon counts. (E) Picture of liver tumor and tumor infiltrating lymphocytes (TILs) detected by flow cytometry. (F) Flow diagram of animal experiment on castrated mice. (G) The luminescence of tumor detected by IVIS system. (H) The statistical results of the animal experiment. (I) Tumor infiltrating lymphocytes (TILs) detected by flow cytometry.