Research Paper Volume 12, Issue 12 pp 11500—11516

Long noncoding RNA LINC00963 induces NOP2 expression by sponging tumor suppressor miR-542-3p to promote metastasis in prostate cancer

Figure 2. Effects of noncoding RNA LINC00963 on PCa cell metastasis in vitro and in vivo. (A) RT-qPCR analysis of LINC00963 expression in the PCa cell lines DU 145 and PC-3 compared to that of the normal prostate cell line RWPE-1. (B) LINC00963 expression in SH-LINC00963 Lentivirus and empty lentivirus vector–treated DU145 cells was tested using RT-qPCR. (C) Transwell invasion assays were performed to determine the invasion of SH-LINC00963 Lentivirus–transfected or empty lentivirus vector–transfected DU 145 cells. (D) A representative of the fluorescence image of LINC00963-GFP expression (green) and DAPI (blue) in SH-LINC00963 lentivirus–infected prostate of PCa mice. Scale bars = 20 μm. (E) RT-qPCR analysis of LINC00963 expression in SH-LINC00963 Lentivirus–transfected or empty lentivirus vector–transfected PCa tissues. (F) Survival time of PCa mice injected with SH-LINC00963 Lentivirus or empty lentivirus vector. (G) Experimental approach to knock down LINC00963 in castrated TRAMP mice. (H) Incidence of TdLN, liver, lung, and bone metastases following injection with SH-LINC00963 Lentivirus or empty lentivirus vector in castrated TRAMP mice (n = 10, every group). (I) The experimental approach to knock down LINC00963 in castrated ProbCre/Ptenfl/fl mice. (J) Incidence of TdLN, liver, lung, and bone metastases following injection with SH-LINC00963 Lentivirus or empty lentivirus vector in castrated ProbCre/Ptenfl/fl mice (n = 10, every group). Mean ± SEM, ****P < 0.001.