Research Paper Volume 12, Issue 12 pp 11500—11516

Long noncoding RNA LINC00963 induces NOP2 expression by sponging tumor suppressor miR-542-3p to promote metastasis in prostate cancer

Figure 5. NOP2 is a target of tumor suppressor miR-542-3p and is suppressed by long noncoding RNA LINC00963 deletion. (A) Venn diagram of intersection target genes of miR-542-3p predicted by several bioinformatics databases. (B) Heat map of top 20 decreased genes in DU 145 cells after transfection with OE-miR-542-3p Lentivirus. (C) Relative expression of NOP2 in PCa tissues compared to that of normal tissue was analyzed by using the TCGA dataset. (D) RT-qPCR analysis of NOP2 expression in PCa cell lines the DU 145 and PC-3 cells compared to the normal prostate cell line RWPE-1. (E) Schematic view of miR-542-3p putative targeting site in the WT and Mut 3’-untranslated region (UTR) of NOP2. (F) Luciferase activity assay in 293 T cells transfected with luciferase report plasmids containing NOP2 3’UTR (WT or Mut), and control miRNA or miR-542-3p. (G) Relative mRNA levels of NOP2 in DU 145 and PC-3 cells transfected with control vector or OE-miR-542-3p Lentivirus. Mean ± SEM, **P < 0.01, ****P < 0.001, NS: no statistical significance.